Based on the comparative genomics study of 13 haemorrhagic septicaemia (HS)-associated strains from Asia and North America with other non-HS associated strains of Pasteurella multocida, unique sequences were identified. One of these was used to develop a rapid (30 minutes) molecular diagnostic test as a candidate for potential deployment in resource and infrastructure-limited countries. The loop mediated isothermal amplification (LAMP) technique allows target DNA amplification under isothermal conditions in a water bath or heat block, thus avoiding the need for a thermal cycler. Results can be visualized by fluorescence under UV light with Syto 9 or Sybr safe dyes. The LAMP test was optimized for magnesium concentration, primer ratio, primer concentration, reaction time and temperature. Performance characteristics of the LAMP test were investigated using 18 DNA mixes of different P.multocida strains and other bacteria at different concentrations. When duplicates of each reaction were run for 30 minutes, the specificity and sensitivity were both 100% for template DNA amounts as low as 5 pg. Alternatively, using a single reaction tube run for 27 minutes, specificity was 92.5% and sensitivity was 96.5% for template DNA amounts down to 5 pg. The next step should be field evaluation of the test in HS-endemic countries like Pakistan and Thailand.