A retrospective epidemiological case-control study was performed in Karachi, Pakistan from January to April 2013. We interviewed 217 dairy cattle and buffalo farms’ owners from 6 different locations around Karachi. The aim of the study was to identify independent variables that were statistically associated with the presence of haemorrhagic septicaemia (HS) on the farm. Farms with a history of at least 1 instance of sudden death in a dairy animal during the year 2012 and a positive clinical HS diagnosis (made by local veterinarians) were considered cases. Farms having no history of sudden death in dairy livestock in 2012 were considered controls. Univariate analysis showed a statistically significant correlation between non-vaccination of the animals and presence of the disease (OR=3.54, 95%CI 1.79-7). Farms having >200 dairy animals were at greater risk of HS outbreaks than farms with <200 head of livestock (OR=2.6, 95%CI 1.27– 5.32) and farms with stocking densities >0.3226/m2 also had an increased risk (OR=4.26, 95%CI 1.06-17.19). Farms using underground water were also at increased risk of HS (OR=2.58, 95%CI 1.27-5.26). These results support the use of animal vaccination to protect dairy animals from HS. Smaller holdings with lower stocking densities also appear to be at lower risk of HS. We are still processing the multivariate analysis and it will be ready by the time of the conference.